coculture相关论文
Due to their special anatomical and physiological features,central nervous system diseases still presented challenges,de......
Differences in phenotype and gene expression of prostate stromal cells from patients of varying ages
Prostate cancer (PCa) is an age-related disease,and the stromal microenvironment plays an important role in prostatic ma......
Nitric oxide induced mechanism(s) regulating biosynthesis of styrylpyrone polyphenols by Basidiomyce
Fungal interspecific interactions enhance biosynthesis of phenylpropanoid metabolites(PM),and production of nitric oxide......
Objective Osteoporosis has become one of the most common skeletal disorders for the elderly, postmenopausal women and th......
Intra-articular chondrogenic activity of bone marrow mesenchymal stem cells co-cultured with allogen
OBJECTIVE:To investigate intra-articular chondrogenic activity of BMSCS co-cultured with allogenicdemineralized bone mat......
Experimental Study on the Repair of Articular Cartilage Defects in the Knee of Wuzhishan Miniature P
Purpose: 1. Investigate the feasibility of construction of tissue engineered cartilage by co-culture of BMSCs and CCs to......
Potential proinflammatory effects of hydroxyapatite nanoparticles on endothelial cells in a monocyte
Objective:Synthetic hydroxyapatite nanoparticles (HANPs) are widely used in nanomedicine fields.The delivery of nanomedi......
We developed a diffused long-term gradient of 293T-producing Slit protein in a micropatterned co-culture system.Using hi......
Experimental study on the induction of bone marrow stromal cells differentiating into cardiomyocyte-
Objective:To investigate the feasibility of bone marrow stromal cells (BMSCs) differentiating into cardiomyocyte-like ce......
目的 探讨心肌细胞对骨髓间充质干细胞(MSCs)向心肌细胞定向诱导分化的作用及其生物学特性.方法 取大鼠四肢骨髓,用细胞分离液通过......
目的 探讨脾对小鼠怀孕状态下胰岛β细胞增加的影响.方法 40只7周龄昆明雌性小鼠分为4组:脾切除怀孕组、假手术怀孕组、未受孕脾切......
目的 研究恒磁场作用对骨髓间充质干细胞(MSCs)向髓核细胞分化的影响.方法 采用4,6-二咪基-2-苯吲哚盐酸(DAPI)标记新西兰大白兔原......
目的 观察胰腺癌细胞株对神经细胞轴突延伸的促进作用,并分析其可能的机制.方法 用倒置显微镜成像系统动态观察胰腺癌细胞与脊髓背......
Experimental study on the induction of bone marrow stromal cells differentiating into cardiomyocyte-
Objective:To investigate the feasibility of bone marrow stromal cells (BMSCs) differentiating into cardiomyocyte-like ce......
Caulerpa okamurae extract attenuates inflammatory interaction,regulates glucose metabolism and incre
Objective:To examine whether Caulerpa okamurae ethanolic extract (COE) could inhibit obesitymediated inflammation,improv......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Production of early monozygotic twin bovineembryos in vitro by the blastomere separation and cocultu
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
目的 探讨大鼠血管内皮祖细胞(endothelial progenitor cell,EPC)对骨髓间充质干细胞(bone marrow mesenchymal stem cell,BMSC)增......
目的 探索细胞外基质(ECM)在猪肝细胞与骨髓间充质干细胞(MSCs)体外共培养中的表达与分布规律. 方法自中华实验猪髂前上棘抽取骨髓......
目的 探讨体外快速培养犬口腔黏膜上皮细胞(OMECs)及其与猪小肠黏膜下层(SIS)体外复合培养的方法,为组织工程化黏膜研究提供实验依......
Osteocytes act as mechanosensors in bone, which can send mechanical signals directly to osteoblasts through gap junction......
A team of researchers from Nagoya, Tokyo and Hamilton developed a unique technique for studying neuro-immune interaction......
AIM: To develop the effective technology for reconstruction of a liver organ in vitro using a bio-artificial liver.METHO......
Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells
Objective To investigate the effect of rat Schwann cell secretion on the proliferation and differentiation of human embr......
目的 比较不同预冻温度及交联方法对胶原膜内部超微结构及成纤维细胞增殖的影响. 方法 将牛Ⅰ型胶原溶液(10 g/L)分别在-20℃和-80......
4~60 μmol/h.培养过程中,仅有少量的酶(AST、LDH)漏出.结论 初步构建的新型多层平板型生物反应器,符合生物人工肝基本的要求.其内......
目的 评价新型多层平板型生物人工肝治疗急性肝功能衰竭动物的疗效.方法 以新鲜猪肝细胞及猪骨髓基质干细胞为细胞来源,共培养于新......
研究了培养条件对玉米渣皮在盘式固态发酵反应器中用AspergillusnigerB11和CandidautilisATCC9256共培养生产细胞白 影响,单因素试验的最佳条件,培养时间60h,培养温度320℃,通风量1.25m^3/min.m^3(WS),料层......
为探讨输卵管上皮细胞对小鼠雌雄原核受精卵(2PN受精卵)体外发育的影响,首选建立了羊输卵管上皮细胞共培养体制,再将小鼠2PN受精卵分别......
目的:体外构建人表皮黑素细胞与角质形成细胞直接接触的共培养模型。方法:胰酶消化包皮的表皮,分别获取纯化的角质形成细胞和黑素细......
BACKGROUND:Stem cells,characterized with convenient collection,wide sources and great potential,have been developed in t......
目的探讨体外诱导人骨髓间充质干细胞(human bone mesenchymal stem cells,HBMSCs)分化为角膜缘干细胞(limbal stem cells,LSCs)的可行......
以脂多糖(LPS)处理复合培养的大鼠肺微血管内皮细胞(LMVEC)和肺动脉平滑肌细胞(PASMC),利用流式细胞仪检测LMVEC和PASMC细胞周期的......
目的:建立成骨细胞和破骨细胞共育的体外模型,为研究骨生理和病理提供新的方法.方法:从胎鼠和4周鼠中分离成骨和破骨细胞,并按照10......
目的:建立兔主动脉内皮细胞(ECs)和平滑肌细胞(SMCs)共培养体系,为进一步开展相关研究打下基础。方法:以Transwell膜为载体,将原代培养的EC......
目的 通过移植骨髓间充质干细胞(mesenchymal stem cell,MSC)的方法试治疗大鼠糖尿病。方法 贴壁生长的MSC与大鼠胰腺的细胞共培养以......
目的建立大鼠脑毛细血管内皮细胞(BCECs)和星形胶质细胞共培养模型,评价纳米粒的跨血脑屏障(BBB)转运和对内皮细胞紧密连接的毒性。方法......
目的:研究高糖时系膜细胞对体外共培养的ECV304细胞产生活性氧和转化生长因子β1(TGF-β1)的影响以及茶多酚的干预作用。方法:建立体外......
采用体外厌氧共培养技术,研究了山羊瘤胃真菌对瘤胃细菌发酵的影响.结果表明:在瘤胃真菌与瘤胃细菌共培养24 h内,瘤胃真菌对瘤胃细......
为进一步完善猪胚胎体外发育系统,以孤雌激活胚胎为研究对象,依次对胚胎培养液种类、培养期间血清添加方式、卵丘细胞共培养体系进......
目的研究脂多糖 (Lipopolysaccharide, LPS) 对复合培养的微血管内皮细胞中Janus激酶(Janus kinase, JAK)基因表达的影响.方法与肺......
目的研究低氧对复合培养的平滑肌细胞中白细胞介素6(IL-6)表达的影响.方法低氧处理与肺微血管内皮细胞复合培养的鼠肺动脉平滑肌细......
目的研究低氧对复合培养的平滑肌细胞中Janus激酶(JAK)基因表达的作用.方法鼠肺微血管内皮细胞与肺动脉平滑肌细胞复合培养,采用半......
哺乳动物缺氧主要通过颈动脉体(CB)化学感受性反射导致肺通气量增加,但感受器细胞释放何种神经递质介导兴奋性化学传递仍存在争议。本......
目的:研究切应力对与血管平滑肌细胞联合培养的内皮细胞的抗凝血和粘附的影响。方法:对与平滑肌细胞联合培养的内皮细胞施加生理范围......
目的:研究切应力作用下与血管平滑肌细胞联合培养的内皮细胞的抗凝血功能。方法:应用流动腔系统,对与平滑肌细胞联合培养的同皮细胞施......
目的应用新生大鼠心肌细胞(CM)与骨髓间充质干细胞(MSCs)体外共培养的方法模拟心肌内环境,研究模拟环境下MSCs分化为CM的相关因素......
以脂多糖(LPS)处理与大鼠肺动脉平滑肌细胞复合培养的肺微血管内皮细胞,测定两种细胞培养上清中白细胞介素6(IL-6)的活性,采用RT-P......
探讨混合表皮细胞与脱细胞真皮体外构建的复合皮作创面移植后的转归.将BALB/c小鼠的表皮细胞和人表皮细胞按一定比例混合,种植于脱......
目的:探讨体外"心肌样"环境对骨髓基质细胞(marrow stromal cells,MSCs)分化的诱导作用.方法:体外将MSCs与心肌细胞共培养至16 d,......